Tumori Journal Abstract Book - October 2020 - 164

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Tumori Journal 106(2S)

months. Median age was 50 (Range: 18-75), M/F ratio was
40(54.8%)/33(45.2%),. MGMT promoter was methylated
in 34 pts (46.6%) and unmethylated in 39 pts (53.4%). After
surgery, 9 pts (12.3%) received RT alone, 57 pts (78.1%)
received both RT and CT (sequential, concomitant or both).
Median survival was 26.2 months. In multivariate analysis
age (HR=1.064, 95%CI: 1.030-1.099; P<0.001) and
MGMT methylation (HR=0.422, 95%CI: 0.210-0.848;
P=0.015) were independently associated with risk for death.
Conclusions: IDH wild type confers a dismal prognosis in
patients with grade III glioma. MGMT methylation, as was
demonstrated in glioblastoma, represents a prognostic factor
that correlated with a reduced risk of death. Further studies
will investigate potential correlations with treatments.

P04
Glucocorticoid Receptors (GR)
upregulation in Circulating
Tumour Cells (CTCs) and Glioma
Stem Cell-like phenotype
(GSCs): role of Dexamethasone
in patients diagnosed with
Glioblastoma Multiforme (GBM)
Spinelli G.P.1, Di Benedetto L.2, Tumminello A.3, Pietranera M.4, Bitca
V.1, Gozzi E.1, Sinjari M.1, Raimondi L.1
1

2

3

UOC Territorial Oncology, Aprilia; BIOS SpA, Roma; Unità Funzionale
Emergenza Sanitaria (UFES), Policlinico Militare Cellio, Roma; 4Centro
Medico Diagnostico Salus, Civitavecchia, Roma

Background: Despite therapeutic improvements,prognosis
in GBM remains extremely poor,for CD133+GSCs too,
responsible for resistant to therapy. Increasing evidence
indicates Dexamethasone(Dex), used to treat peritumoral
GBM-associated oedema, could be linked to cell growth
and poor prognosis in different tumours but its role in
GBM has remained controversial. In our study, we investigated the expression of GR in CTCs from cerebrospinal
fluid (CSF) of pts with GBM and we analyzed the potential correlation between Dex treatment and the higher
CD133+CTCs levels.
Methods: Serial matched blood and lumbar puncture samples were collected from 20 pts with GBM at day 0(before
surgery) and every 2/4 months until progression or death.
CTCs from CSF were evaluated by CellSearch system and
correlated with citology: Low and High density neutrophils (LDNs and HDNs, respectively) levels and their ratio
were determined. GR was assessed on CTCs; flow cytometry and uni-multivariate analysis were performed to detect
CD133+ and to correlate cellular subpopulations with OS.
Results: In all pts, MGMT methylation and IDH1/2 status
were detected. All samples contained CTCs; at baseline
mean CTCs was 112 per 3ml (range 4-350) and 9/20 pts
had GR negative CTCs. All samples had CD133+CTCs

(median 17.9%±10.1; range: 2- 31%). For symptom control, all patients received Dex at diagnosis; different doses
of Dex were administered. Change in GR CTCs count
occurred with higher Dex doses: 5/9 pts changed their GR
status from negative to positive and pts with pre-Dex positive GR CTCs, upregulated their GR levels (p<0.001). A
significantly correlation between CD133+CTCs and GR
upregulation was observed (p<0.001). After having taken
Dex, median CTCs number was 301 (range: 87-521) and
CD133+ cells were 72.6% (range: 10-97%, p<0.001).
Analyzing LDNs/HDNs, we demonstrated an increased
ratio in pts with upregulated GR and elevated CD133+CTCs
(0.581 ± 0.102 vs. 0.041 ± 0.018, p=0.001). All these data
were associated with pts' outcome: median PFS and OS in
the subgroup with GR-positive CTCs were significantly
shorter (5months and 12months, respectively) than in the
subgroup with GR-negative (18months and 51months,
respectively), regardless of MGMT and IDH1/2 status
(p = 0.002 and p<0.001).
Conclusions: GBM remains a crucial public health issue.
Survival analyses are consistent with the hypothesis that
treatment of GBM with the highest dose of Dex could promote changes in GR CTCs enumeration, contributing to
development of GSC-like population and poor prognosis.

P05
MET-expression in glioblastoma, a
new prognostic factor?
Caserta C.1, Mosillo C.1, Guida A.1, Macrini S.1, Peciarolo A.2,
Calandrella M.L.1, Mannino A.1, Ascani S.2, Bracarda S.2
1
Medical and Translational Oncology Unit, Azienda Ospedaliera S. Maria,
Terni, Italy; 2Pathology Unit, Azienda Ospedaliera Santa Maria di Terni,
University of Perugia, Terni, Italy

Background: Glioblastoma (GBM) is the most common
malignant primary brain tumor. Despite the use of intensive treatment, GBM still has a poor prognosis (15-18
months). MET overexpression was described in GBM and
often relates with worse survival. The aim of our study is
to investigate MET expression rate, its prognostic role, and
potential correlations with other biomarkers.
Material and methods: Retrospective study on patients
(pts) affected by GBM treated at S. Maria Hospital of Terni
from Jan 2015 to Dec 2019. Pts were managed according to
established diagnostic and therapeutic protocols. Clinical
informations were collected from pts' files. MET expression was evaluated by immunohistochemistry using
CONFIRM anti-total MET (SP44) rabbit monoclonal antibody. The proportions of positively stained tumor cells
were scored as follows: 0) no positive tumor cells; 1)
<10%; 2) 10-30%; 3) >30%. Spearman's and Chi's square
tests were used to define the correlation and the difference
in expression of different tumor markers, respectively. The



Tumori Journal Abstract Book - October 2020

Table of Contents for the Digital Edition of Tumori Journal Abstract Book - October 2020

Contents
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Tumori Journal Abstract Book - October 2020 - Cover2
Tumori Journal Abstract Book - October 2020 - I
Tumori Journal Abstract Book - October 2020 - II
Tumori Journal Abstract Book - October 2020 - Contents
Tumori Journal Abstract Book - October 2020 - IV
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