Tumori Journal Abstract Book - October 2020 - 163

P - Brain Tumours	
Conclusions: Our study demonstrated poor benefit of pembrolizumab in recurrent HGG patients with immunohistochemical loss of MMR proteins. No molecular biomarker
was found to be associated to pembrolizumab activity.

P02
Defining the prognostic role
of MGMT methylation value
by pyrosequencing assay in
glioblastoma patients: A large
Italian multicenter study
Caccese M.1, Simonelli M.2, Bellu L.3, Villani V.4, Rizzato S.5, Ius T.6,
Pasqualetti F.7, Russo M.8, Franchino F.9, Amoroso R.10, Bertorelle
R.11, Cavallin F.12, Dipasquale A.2, Carosi M.13, Pizzolitto S.14, Cesselli
D.15, Gardiman M.P.16, Padovan M.17, Zagonel V.17, Lombardi G.17
1

Department of Oncology, Oncology 1, Veneto Institute of Oncology IOV IRCCS - Padova, Padova; 2Humanitas Cancer Center; Humanitas University,
Department of Biomedical Sciences, Milano; 3Radiotherapy Unit, Veneto
Institute of Oncology IOV-IRCCS, Padova; 4Neuro-Oncology Unit, Regina Elena
National Cancer Institute, Roma; 5Department of Oncology, Azienda Sanitaria
Universitaria Friuli Centrale, Udine; 6Department of Neurosurgery, University
Hospital of Udine, Udine; 7Radiation Oncology, Pisa University Hospital,
Pisa; 8Neurology Unit, Azienda USL-IRCCS di Reggio Emilia, Reggio Emilia;
9
Department of Neuro-Oncology, University of Turin and City of Health and
Science Hospital, Torino; 10Divisione di Neurochirurgia, Azienda Sanitaria
Alto Adige-Comprensorio Sanitario di Bolzano, Bolzano; 11Immunology
and Molecular Oncology, Veneto Institute of Oncology-IRCCS, Padova;
12
Independent Statistician, Solagna; 13Pathology Unit, Regina Elena National
Cancer Institute, Roma; 14Department of Surgical Pathology, Azienda Sanitaria
Universitaria Friuli Centrale, Udine; 15Department of Medicine, University of
Udine; Institute of Pathology, Azienda Sanitaria Universitaria Friuli Centrale,
Udine; 16Department of Medicine and Surgical Pathology and Cytopahology
Unit; University Hospital of Padua, Padova; 17Department of Oncology,
Oncology 1, Veneto Institute of Oncology IOV-IRCCS, Padova

Background: MGMT methylation (MGMTmet) status
represents an important prognostic factor for glioblastoma
(GBM) patients (PTS). Quantitative pyrosequencing
approach has proven to be feasible for MGMTmet testing
but its value is still unclear. We performed a large, multicentre, retrospective study to identify the association
between MGMTmet values and clinical outcome.
Material and methods: From 9 Italian neuro-oncology
centres, we collected consecutive GBM PTS with assessment of MGMTmet by pyrosequencing approach evaluating CpG islands from 75 to 84. Other inclusion criteria
were: histological diagnosis of GBM, ECOG PS =2, therapy
with RT+TMZ. Kaplan-Meier method was used to estimate
the survival curves, time-dependent ROC curve for defining
the optimal cut-off value of mean percentage of MGMTmet
in terms of 2y-OS, Cox regression for multivariable analysis, and restricted cubic spline to investigate the non-linear
association between methylation values and OS.
Results: 681 PTS were enrolled; median age was 60 ys;
ECOG PS was 0 in 292 PTS, 1 in 306 PTS, 2 in 83 PTS;
391 PTS (58%) had a complete resection. 8% of PTS

163
received a second surgery. IDH was mutated in 6%. 2y-OS
was 31.6%, median OS was 17.4 ms. Median MGMTmet
was 3.5% (IQR 0-22%). ROC curve identified a cutoff of
15% of MGMTmet in terms of 2y-OS (sens 78%, spec
57%, AUC = 0.67). 2y-OS was 19.7% and 53.7% for PTS
with MGMTmet < and =15%, respectively (p < 0.0001).
At multivariable analysis, MGMTmet < 15% was associated with impaired survival (HR 2.7, 95%CI 2.1-3.4; p <
0.00001), adjusting for age, KPS, type of surgery and second surgery. A non-linear association between MGMT
methylation and survival was identified (non-linear term:
p < 0.0001), with lower values of MGMT methylation
associated with lower survival; indeed, estimated median
OS was lowest (14 months, 2ys-OS: 17.4%) with
MGMTmet of 4%, 21ms (2yr-OS: 40.9%) with MGMTmet
of 20%, 27ms (2yr-OS: 40.9%) when MGMTmet was
40%, then leveled around 30ms (2yr-OS: 54.5-59.8%)
when MGMTmet was > 40%.
Conclusions: This study represents one of the largest trials
analyzing MGMTmet by pyrosequencing approach. Lower
values of MGMTmet were associated with impaired survival
and the relationship was non-linear. Noteworthy, we identified a strong prognostic value of MGMTmet which could be
used as stratification factor in prospective clinical trials.

P03
MGMT status influences prognosis
of patients with IDH wild type
grade III gliomas
Di Nunno V.1, Franceschi E.2, Tosoni A.2, Gatto L.2, Mura A.2,
Minichillo S.2, Di Battista M.2, Lamperini C.2, Nuvola G.3, Sisi M.3,
Mosca M.3, Bartolini S.2, Brandes A.A.2
1

Department of Medical Oncology, Azienda USL-IRCCS Institute of
Neurological Sciences,, Bologna; 2Department of Medical Oncology, Azienda
USL-IRCCS Institute of Neurological Sciences, Bologna, Italy., Bologna;
3
Department of Oncology Sant'Orsola Malpghi Hospital - University of
Bologna, Bologna

Background: WHO grade III gliomas are classified
according to the presence of IDHmutation. IDH wild type
(IDH wt) is associated with poor prognosis and limited
effectiveness of treatments. The aim of this study was to
find out if MGMT methylation represents a prognostic factor in this setting.
Methods: We analyzed our Institutional data warehouse
for all consecutive patients (pts) with newly diagnosed,
histologically proven grade III, IDH wt gliomas. The IDH
1/2 assessment was performed by Next Generation
Sequencing (NGS). MGMT methylation was assessed by
methylation specific PCR (MSP). Tissue samples were
also centrally reviewed for histology.
Results: The analysis included 73 pts with grade III, IDH
wt (19.3%) gliomas. The median follow-up time was 69.9



Tumori Journal Abstract Book - October 2020

Table of Contents for the Digital Edition of Tumori Journal Abstract Book - October 2020

Contents
Tumori Journal Abstract Book - October 2020 - Cover1
Tumori Journal Abstract Book - October 2020 - Cover2
Tumori Journal Abstract Book - October 2020 - I
Tumori Journal Abstract Book - October 2020 - II
Tumori Journal Abstract Book - October 2020 - Contents
Tumori Journal Abstract Book - October 2020 - IV
Tumori Journal Abstract Book - October 2020 - V
Tumori Journal Abstract Book - October 2020 - 1
Tumori Journal Abstract Book - October 2020 - 2
Tumori Journal Abstract Book - October 2020 - 3
Tumori Journal Abstract Book - October 2020 - 4
Tumori Journal Abstract Book - October 2020 - 5
Tumori Journal Abstract Book - October 2020 - 6
Tumori Journal Abstract Book - October 2020 - 7
Tumori Journal Abstract Book - October 2020 - 8
Tumori Journal Abstract Book - October 2020 - 9
Tumori Journal Abstract Book - October 2020 - 10
Tumori Journal Abstract Book - October 2020 - 11
Tumori Journal Abstract Book - October 2020 - 12
Tumori Journal Abstract Book - October 2020 - 13
Tumori Journal Abstract Book - October 2020 - 14
Tumori Journal Abstract Book - October 2020 - 15
Tumori Journal Abstract Book - October 2020 - 16
Tumori Journal Abstract Book - October 2020 - 17
Tumori Journal Abstract Book - October 2020 - 18
Tumori Journal Abstract Book - October 2020 - 19
Tumori Journal Abstract Book - October 2020 - 20
Tumori Journal Abstract Book - October 2020 - 21
Tumori Journal Abstract Book - October 2020 - 22
Tumori Journal Abstract Book - October 2020 - 23
Tumori Journal Abstract Book - October 2020 - 24
Tumori Journal Abstract Book - October 2020 - 25
Tumori Journal Abstract Book - October 2020 - 26
Tumori Journal Abstract Book - October 2020 - 27
Tumori Journal Abstract Book - October 2020 - 28
Tumori Journal Abstract Book - October 2020 - 29
Tumori Journal Abstract Book - October 2020 - 30
Tumori Journal Abstract Book - October 2020 - 31
Tumori Journal Abstract Book - October 2020 - 32
Tumori Journal Abstract Book - October 2020 - 33
Tumori Journal Abstract Book - October 2020 - 34
Tumori Journal Abstract Book - October 2020 - 35
Tumori Journal Abstract Book - October 2020 - 36
Tumori Journal Abstract Book - October 2020 - 37
Tumori Journal Abstract Book - October 2020 - 38
Tumori Journal Abstract Book - October 2020 - 39
Tumori Journal Abstract Book - October 2020 - 40
Tumori Journal Abstract Book - October 2020 - 41
Tumori Journal Abstract Book - October 2020 - 42
Tumori Journal Abstract Book - October 2020 - 43
Tumori Journal Abstract Book - October 2020 - 44
Tumori Journal Abstract Book - October 2020 - 45
Tumori Journal Abstract Book - October 2020 - 46
Tumori Journal Abstract Book - October 2020 - 47
Tumori Journal Abstract Book - October 2020 - 48
Tumori Journal Abstract Book - October 2020 - 49
Tumori Journal Abstract Book - October 2020 - 50
Tumori Journal Abstract Book - October 2020 - 51
Tumori Journal Abstract Book - October 2020 - 52
Tumori Journal Abstract Book - October 2020 - 53
Tumori Journal Abstract Book - October 2020 - 54
Tumori Journal Abstract Book - October 2020 - 55
Tumori Journal Abstract Book - October 2020 - 56
Tumori Journal Abstract Book - October 2020 - 57
Tumori Journal Abstract Book - October 2020 - 58
Tumori Journal Abstract Book - October 2020 - 59
Tumori Journal Abstract Book - October 2020 - 60
Tumori Journal Abstract Book - October 2020 - 61
Tumori Journal Abstract Book - October 2020 - 62
Tumori Journal Abstract Book - October 2020 - 63
Tumori Journal Abstract Book - October 2020 - 64
Tumori Journal Abstract Book - October 2020 - 65
Tumori Journal Abstract Book - October 2020 - 66
Tumori Journal Abstract Book - October 2020 - 67
Tumori Journal Abstract Book - October 2020 - 68
Tumori Journal Abstract Book - October 2020 - 69
Tumori Journal Abstract Book - October 2020 - 70
Tumori Journal Abstract Book - October 2020 - 71
Tumori Journal Abstract Book - October 2020 - 72
Tumori Journal Abstract Book - October 2020 - 73
Tumori Journal Abstract Book - October 2020 - 74
Tumori Journal Abstract Book - October 2020 - 75
Tumori Journal Abstract Book - October 2020 - 76
Tumori Journal Abstract Book - October 2020 - 77
Tumori Journal Abstract Book - October 2020 - 78
Tumori Journal Abstract Book - October 2020 - 79
Tumori Journal Abstract Book - October 2020 - 80
Tumori Journal Abstract Book - October 2020 - 81
Tumori Journal Abstract Book - October 2020 - 82
Tumori Journal Abstract Book - October 2020 - 83
Tumori Journal Abstract Book - October 2020 - 84
Tumori Journal Abstract Book - October 2020 - 85
Tumori Journal Abstract Book - October 2020 - 86
Tumori Journal Abstract Book - October 2020 - 87
Tumori Journal Abstract Book - October 2020 - 88
Tumori Journal Abstract Book - October 2020 - 89
Tumori Journal Abstract Book - October 2020 - 90
Tumori Journal Abstract Book - October 2020 - 91
Tumori Journal Abstract Book - October 2020 - 92
Tumori Journal Abstract Book - October 2020 - 93
Tumori Journal Abstract Book - October 2020 - 94
Tumori Journal Abstract Book - October 2020 - 95
Tumori Journal Abstract Book - October 2020 - 96
Tumori Journal Abstract Book - October 2020 - 97
Tumori Journal Abstract Book - October 2020 - 98
Tumori Journal Abstract Book - October 2020 - 99
Tumori Journal Abstract Book - October 2020 - 100
Tumori Journal Abstract Book - October 2020 - 101
Tumori Journal Abstract Book - October 2020 - 102
Tumori Journal Abstract Book - October 2020 - 103
Tumori Journal Abstract Book - October 2020 - 104
Tumori Journal Abstract Book - October 2020 - 105
Tumori Journal Abstract Book - October 2020 - 106
Tumori Journal Abstract Book - October 2020 - 107
Tumori Journal Abstract Book - October 2020 - 108
Tumori Journal Abstract Book - October 2020 - 109
Tumori Journal Abstract Book - October 2020 - 110
Tumori Journal Abstract Book - October 2020 - 111
Tumori Journal Abstract Book - October 2020 - 112
Tumori Journal Abstract Book - October 2020 - 113
Tumori Journal Abstract Book - October 2020 - 114
Tumori Journal Abstract Book - October 2020 - 115
Tumori Journal Abstract Book - October 2020 - 116
Tumori Journal Abstract Book - October 2020 - 117
Tumori Journal Abstract Book - October 2020 - 118
Tumori Journal Abstract Book - October 2020 - 119
Tumori Journal Abstract Book - October 2020 - 120
Tumori Journal Abstract Book - October 2020 - 121
Tumori Journal Abstract Book - October 2020 - 122
Tumori Journal Abstract Book - October 2020 - 123
Tumori Journal Abstract Book - October 2020 - 124
Tumori Journal Abstract Book - October 2020 - 125
Tumori Journal Abstract Book - October 2020 - 126
Tumori Journal Abstract Book - October 2020 - 127
Tumori Journal Abstract Book - October 2020 - 128
Tumori Journal Abstract Book - October 2020 - 129
Tumori Journal Abstract Book - October 2020 - 130
Tumori Journal Abstract Book - October 2020 - 131
Tumori Journal Abstract Book - October 2020 - 132
Tumori Journal Abstract Book - October 2020 - 133
Tumori Journal Abstract Book - October 2020 - 134
Tumori Journal Abstract Book - October 2020 - 135
Tumori Journal Abstract Book - October 2020 - 136
Tumori Journal Abstract Book - October 2020 - 137
Tumori Journal Abstract Book - October 2020 - 138
Tumori Journal Abstract Book - October 2020 - 139
Tumori Journal Abstract Book - October 2020 - 140
Tumori Journal Abstract Book - October 2020 - 141
Tumori Journal Abstract Book - October 2020 - 142
Tumori Journal Abstract Book - October 2020 - 143
Tumori Journal Abstract Book - October 2020 - 144
Tumori Journal Abstract Book - October 2020 - 145
Tumori Journal Abstract Book - October 2020 - 146
Tumori Journal Abstract Book - October 2020 - 147
Tumori Journal Abstract Book - October 2020 - 148
Tumori Journal Abstract Book - October 2020 - 149
Tumori Journal Abstract Book - October 2020 - 150
Tumori Journal Abstract Book - October 2020 - 151
Tumori Journal Abstract Book - October 2020 - 152
Tumori Journal Abstract Book - October 2020 - 153
Tumori Journal Abstract Book - October 2020 - 154
Tumori Journal Abstract Book - October 2020 - 155
Tumori Journal Abstract Book - October 2020 - 156
Tumori Journal Abstract Book - October 2020 - 157
Tumori Journal Abstract Book - October 2020 - 158
Tumori Journal Abstract Book - October 2020 - 159
Tumori Journal Abstract Book - October 2020 - 160
Tumori Journal Abstract Book - October 2020 - 161
Tumori Journal Abstract Book - October 2020 - 162
Tumori Journal Abstract Book - October 2020 - 163
Tumori Journal Abstract Book - October 2020 - 164
Tumori Journal Abstract Book - October 2020 - 165
Tumori Journal Abstract Book - October 2020 - 166
Tumori Journal Abstract Book - October 2020 - 167
Tumori Journal Abstract Book - October 2020 - 168
Tumori Journal Abstract Book - October 2020 - 169
Tumori Journal Abstract Book - October 2020 - 170
Tumori Journal Abstract Book - October 2020 - 171
Tumori Journal Abstract Book - October 2020 - 172
Tumori Journal Abstract Book - October 2020 - 173
Tumori Journal Abstract Book - October 2020 - 174
Tumori Journal Abstract Book - October 2020 - 175
Tumori Journal Abstract Book - October 2020 - 176
Tumori Journal Abstract Book - October 2020 - 177
Tumori Journal Abstract Book - October 2020 - 178
Tumori Journal Abstract Book - October 2020 - 179
Tumori Journal Abstract Book - October 2020 - 180
Tumori Journal Abstract Book - October 2020 - 181
Tumori Journal Abstract Book - October 2020 - 182
Tumori Journal Abstract Book - October 2020 - 183
Tumori Journal Abstract Book - October 2020 - 184
Tumori Journal Abstract Book - October 2020 - 185
Tumori Journal Abstract Book - October 2020 - 186
Tumori Journal Abstract Book - October 2020 - 187
Tumori Journal Abstract Book - October 2020 - 188
Tumori Journal Abstract Book - October 2020 - 189
Tumori Journal Abstract Book - October 2020 - 190
Tumori Journal Abstract Book - October 2020 - 191
Tumori Journal Abstract Book - October 2020 - 192
Tumori Journal Abstract Book - October 2020 - 193
Tumori Journal Abstract Book - October 2020 - 194
Tumori Journal Abstract Book - October 2020 - 195
Tumori Journal Abstract Book - October 2020 - 196
Tumori Journal Abstract Book - October 2020 - 197
Tumori Journal Abstract Book - October 2020 - 198
Tumori Journal Abstract Book - October 2020 - 199
Tumori Journal Abstract Book - October 2020 - 200
Tumori Journal Abstract Book - October 2020 - 201
Tumori Journal Abstract Book - October 2020 - 202
Tumori Journal Abstract Book - October 2020 - 203
Tumori Journal Abstract Book - October 2020 - 204
Tumori Journal Abstract Book - October 2020 - 205
Tumori Journal Abstract Book - October 2020 - 206
Tumori Journal Abstract Book - October 2020 - 207
Tumori Journal Abstract Book - October 2020 - 208
Tumori Journal Abstract Book - October 2020 - 209
Tumori Journal Abstract Book - October 2020 - 210
Tumori Journal Abstract Book - October 2020 - 211
Tumori Journal Abstract Book - October 2020 - 212
Tumori Journal Abstract Book - October 2020 - 213
Tumori Journal Abstract Book - October 2020 - 214
Tumori Journal Abstract Book - October 2020 - 215
Tumori Journal Abstract Book - October 2020 - 216
Tumori Journal Abstract Book - October 2020 - 217
Tumori Journal Abstract Book - October 2020 - 218
Tumori Journal Abstract Book - October 2020 - 219
Tumori Journal Abstract Book - October 2020 - 220
Tumori Journal Abstract Book - October 2020 - 221
Tumori Journal Abstract Book - October 2020 - 222
Tumori Journal Abstract Book - October 2020 - 223
Tumori Journal Abstract Book - October 2020 - 224
Tumori Journal Abstract Book - October 2020 - 225
Tumori Journal Abstract Book - October 2020 - 226
Tumori Journal Abstract Book - October 2020 - 227
Tumori Journal Abstract Book - October 2020 - 228
Tumori Journal Abstract Book - October 2020 - 229
Tumori Journal Abstract Book - October 2020 - 230
Tumori Journal Abstract Book - October 2020 - 231
Tumori Journal Abstract Book - October 2020 - 232
Tumori Journal Abstract Book - October 2020 - 233
Tumori Journal Abstract Book - October 2020 - 234
Tumori Journal Abstract Book - October 2020 - 235
Tumori Journal Abstract Book - October 2020 - Cover3
Tumori Journal Abstract Book - October 2020 - Cover4
https://www.nxtbookmedia.com