Tumori Journal Abstract Book - October 2020 - 153

M - Melanoma and Skin Cancers	
Conclusions: A thyroid nodule in a patient with a history
of RCC should be considered as potentially metastatic. It's
impossible distinguish between primary and secondary
thyroid neoplasms on imaging, in fact clinical manifestation and radiographic findings are nonspecific. FNA cytology and immunohistochemistry are helpful in establishing
diagnosis and should be obtained in suspected cases.
Definitive diagnosis of metastatic RCC is usually made by
histopathological examination after thyroidectomy. Our
cases demonstrate the importance of considering RCC
metastasis to the thyroid even years after nephrectomy to
decrease potential delays in diagnosis.

153
uncertain/unknown significance variants. Considering the
global cohort, the consistency in pathogenic/pathogenic
like mutations was 76%. Consistency for BRAF and
NRAS mutations was 95.2% and 85.7% respectively,
without statistically significant differences between the
discovery and validation cohort.
Conclusions: Our study showed a high level of concordance in mutational patterns between primary and metastatic CMM, especially when pathogenic mutations in
driver genes were considered.

M02
M - Melanoma and Skin Cancers
M01
Concordance of mutation
patterns between primary and
metastatic melanoma through
a next-generation sequencing
assay
Manca A.1, Paliogiannis P.2, Lissia A.2, Cossu A.2, Casula M.3,
Colombino M.3, Sini M.C.3, Palomba G.3, Rozzo C.1, Pisano M.1,
Team M.U.S.2, Palmieri G.1
1

IRGB-CNR, Sassari; 2AOU, Sassari; 3ICB-CNR, Sassari

Background: Cutaneous malignant melanoma (CMM) is
one of the most common skin cancers worldwide. Limited
information is available in the current scientific literature
on the concordance of genetic alterations between primary
and metastatic CMM. In the present study, we performed
next-generation sequencing (NGS) analysis of the main
genes participating in melanoma pathogenesis and progression, among paired primary and metastatic lesions of
CMM patients, with the aim to evaluate levels of discrepancies in mutational patterns.
Methods: Paraffin-embedded tumor tissues of the paired
lesions were retrieved from the archives of the institutions
participating in the study. NGS was performed using a specific multiple-gene panel constructed by the Italian
Melanoma Intergroup (IMI) to explore the mutational status of selected regions (343 amplicons; amplicon range:
125-175 bp; coverage 100%) within the main 25 genes
involved in CMM pathogenesis; sequencing was performed with the Ion Torrent PGM System.
Results: A discovery cohort encompassing 30 cases, and a
validation cohort including eleven Sardinian patients with
tissue availability from both the primary and metachronous metastatic lesions were identified; the global number
of analyzed tissue specimens was 90. A total of 829 genetic
non-synonymous variants were detected: 101 (12.2%)
were pathogenic/likely pathogenic, 131 (15.8%) were
benign/likely benign, and the remaining 597 (72%) were

Role of eosinophilia as prognostic
biomarker in metastatic melanoma
(MM) patients receiving anti-PD1
inhibitors: a retrospective single
institution analysis
Conca R.1, Zoppoli P.2, Laurino S.2, Aieta M.1, Passarelli A.1
1
Unit of Medical Oncology, Department of Onco-Hematology. IRCCS-CROB
Referral Cancer Center of Basilicata. Rionero in Vulture (PZ), Italy, Rionero
in Vulture (PZ); 2Laboratory of Preclinical and Translational Research. IRCCSCROB Referral Cancer Center of Basilicata. Rionero in Vulture (PZ), Italy,
Rionero in Vulture (PZ)

Background: Eosinophilia has been identified as a prognostic marker in immunotherapy of MM and suggested to
contribute to anti-tumor host defense. Nevertheless, the
influence of anti-PD1 monoclonal antibodies (mAbs) on
eosinophils (Eos) is poorly studied. Recently, it has been
reported that early increase of Eos in peripheral blood
could be associated with immunotherapy efficacy in MM.
Patients and methods: We retrospectively evaluated clinical data and Eos count of 28 cutaneous MM patients (pts)
receiving anti-PD1 mAbs from January 2018 to April
2020. Eos peripheral count was evaluated at different
time-points: baseline (T0), at second infusion (T1) and at
first tumor assessment (T2). Baseline Eos count and its
variation during treatment were analyzed for the association with efficacy (responders group versus non-responders group), and survival (overall survival [OS]). Log-rank
test and Cox proportional hazards identified the eosinophilia role on OS. Data were analyzed using R software.
Results: In our cohort 14 patients were male (50%) and
median age was 68 years. Stage classification was III,
M1a, M1b, M1c, and M1d in 2 (7.1%), 7 (25%), 6 (21.3%),
10 (35.8%) and 3 (10.8%) pts, respectively. BRAF was
mutated in 4 (15%) pts. 9 patients (32%) received prior
therapy for MM. A clinical response occurred in 21 patients
(75%; responders group) and included 2 with complete
response, 10 with partial response and 9 with stable disease, whereas progressive disease was observed in 7
patients (25%; non-responders group). The difference of
Eos count (defined as DEos) between T1-T0 and T2-T0



Tumori Journal Abstract Book - October 2020

Table of Contents for the Digital Edition of Tumori Journal Abstract Book - October 2020

Contents
Tumori Journal Abstract Book - October 2020 - Cover1
Tumori Journal Abstract Book - October 2020 - Cover2
Tumori Journal Abstract Book - October 2020 - I
Tumori Journal Abstract Book - October 2020 - II
Tumori Journal Abstract Book - October 2020 - Contents
Tumori Journal Abstract Book - October 2020 - IV
Tumori Journal Abstract Book - October 2020 - V
Tumori Journal Abstract Book - October 2020 - 1
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Tumori Journal Abstract Book - October 2020 - Cover3
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