Tumori Journal Abstract Book - October 2020 - 148

148	
diagnosis (yes vs no, p=0,158), Heng and MSKCC prognostic scores (good vs other, p=0,945 and p=0,444, respectively) and number of lines of therapies received (⩾3 vs
<3, p=0,110). The occurrence of lung, bone, lymph-nodes
and central nervous system (CNS) metastases was similar
among the two groups (p=ns), while liver metastases were
higher in elderly pts (p=0,006). Elderly pts received more
pazopanib as I line tyrosine-kinase therapy than youngers
(p=0,0026). Median progression-free survival (mPFS) for
first-line therapy was similar (8,6 and 8,5 months in youngers and elderly respectively, p=0,117). Globally, median
OS (mOS) was 27 months (CI 95% 20,9-38,2), with no
significant difference among youngers and elderly: 27,4
(CI 95% 16,3-40,4) and 24,1 months (CI 95%, 12,5-61,3),
p=0,418. Interestingly, among youngers mOS was significantly different between pts with metastatic disease at
diagnosis and pts relapsed (11,3 vs 44 months, p=0,0004),
but not among elderly pts (23,9 vs 24,2 months).
Conclusions: Despite elderly being more fragile pts
because of their greater comorbidities, age seems not to
impact on survival outcomes in our small series. Therefore,
considering the overall increase in life expectancy, studies
aimed at the specific evaluation of elderly population are
eagerly awaited in order to define the best therapeutic
strategy and the biological characteristics of their disease.

H21
PROSTATE CANCER ASSOCIATED NK
CELLS ARE ENDOWED WITH PROANGIOGENIC PHENOTYPE/FUNCTIONS
AND INDUCE M2-LIKE MACROPHAGE
POLARIZATION

Tumori Journal 106(2S)
(FC) for surface antigens on peripheral blood samples PCa
patients. Conditioned media (CM) from FACS-sorted
PTANKs were used for functional studies of angiogenesis,
on human umbilical-vein endothelial cells (HUVEC),
studies for macrophage recruitment (migration assay on
Boyden chambers) and polarization. Molecular studies
were performed by real time PCR (qPCR) on HUCECs
and macrophages exposed to CM of PTANKs. Protein
arrays were performed to characterize the secretome on
FACS-sorted PTANKs.
Results: We found that PTANKs acquire the pro-angiogenic/decidual-like
CD56brightCD9+CD49a+CXCR4+
phenotype. The same phenotype was observed on cytolytic
NK cells, from healthy donors, exposed to CMs of three
different PCa cell lines. These results were confirmed also
exposing heathy-donor derived NKs to CMs of 3 different
prostate cancer cell lines (PC-3, DU-145, LNCaP),
together with increased production of CXCL8, Angiogenin,
Angiopoietin1 and reduced production of TNFa, IFNg and
GranzymeA. CMs from PTANKs support the formation on
capillary-like structures on HUVEC, together with
increased expression of VEGF, VEGR-2, CXCL8,
ICAM-1 and VCAM-1. Secretome analysis revealed the
ability of PTANKs release pro-angiogenic factors (CLXL8,
MMP-1, MMP-9; uPAR) and cytokines/chemokines
involved in macrophage recruitment (CCL1, CCL2,
CCL5, CCL7, CCL13, CXCL1, CXCL11) and M2-like
polarization (IL-10). Finally, CMs from PTANKs can
recruit THP-1 monocyte and polarize THP-1 macrophage
towards CD206, Arginase1, CXCL8-expressing M2-like/
TAM phenotype.
Conclusions: Our results place PTANKS as effector cells
able in supporting angiogenesis in PCa by directly interaction with endothelial cells and via macrophage polarization.

Albini A.1, Gallazzi M.2, Baci D.2, Naselli A.1, Guarnieri A.1, Mortara
L.2, Deho F.3, Noonan D.4, Bruno A.4
1

IRCCS MultiMedica, Milano; 2Department Biotechnologies and Biosciences,
University of Insubria, Varese; 3S.C. of Urology, ASST Settelaghi, Ospedale di
Circolo e Fondazione Macchi, Varese; 4Science and Technology Park (PST),
IRCCS MultiMedica, Milano

Background: Prostate cancer (PCa) remains the second
most common cancer worldwide in men. Natural killer
(NK) cells, effector lymphocytes of the innate immunity,
have been found to be compromised in solid cancers,
including PCa. We demonstrated that NK cells in lung and
colorectal cancer, acquire a pro-angiogenic phenotype,
similar to NK cell within the decidua (dNK), and functionally support angiogenesis, via TGFb and Stat3/Stat-5 activation. Here, we phenotypically and functionally
characterized circulating NK cells from PCa patients
(PTANKs) and investigated their interaction with endothelial cells and macrophages.
Material (patients) and methods: NK cell subset distribution was investigated by multicolor flow cytometry

H22
Long-term survival of
patients with metastatic
renal cell carcinoma (mRCC): a
retrospective analysis of disease
characteristics
Fanelli M.1, Vitale M.G.1, Caputo F.1, Cerma K.1, Bardasi C.1, Riggi
M.L.1, Baldessari C.1, Dominici M.1, Sabbatini R.1
1

University Hospital of Modena, Division of Oncology, Modena, Modena

Background: Targeted therapy and immunotherapy have
improved clinical outcomes of patients (pts) with metastatic
renal cell carcinoma (mRCC) recently, resulting in median
overall survival (mOS) around 25 months (mos). However,
a subgroup of pts draws scarce benefit from these therapies.
In this retrospective study, we evaluated the disease characteristics potentially related to long- and short-term survival.



Tumori Journal Abstract Book - October 2020

Table of Contents for the Digital Edition of Tumori Journal Abstract Book - October 2020

Contents
Tumori Journal Abstract Book - October 2020 - Cover1
Tumori Journal Abstract Book - October 2020 - Cover2
Tumori Journal Abstract Book - October 2020 - I
Tumori Journal Abstract Book - October 2020 - II
Tumori Journal Abstract Book - October 2020 - Contents
Tumori Journal Abstract Book - October 2020 - IV
Tumori Journal Abstract Book - October 2020 - V
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