Tumori Journal Abstract Book - October 2020 - 128

128	
was no apparent difference in BRCA gene or mutation
location for long- vs short-term responders. Nine of the 11
long-term responders with BRCA wild-type OC had high
genome-wide LOH (⩾16% LOH) including OC associated with deleterious RAD51C/D mutations (n=2).
Conclusions: Long-term responders to rucaparib include
OC with BRCA mutation, particularly homozygous deletion or rearrangements, which would not be susceptible to
somatic reversion mutations, and RAD51C/D mutations.

F02
CORRELATION BETWEEN miRNA
EXPRESSION PATTERN AND PLATINUM
SENSITIVITY IN HIGH GRADE SEROUS
OVARIAN CANCER: A MONOCENTRIC
RETROSPECTIVE EXPERIENCE
Lancia F.1, Mauro E.1, Tiberi E.1, Trimarchi E.1, Marzola M.1, Gafà
R.1, Negrini M.1, Frassoldati A.1
1

Nuovo Arcispedale Sant'Anna, Ferrara

Background: Despite the therapeutic innovations of
recent years, ovarian cancer remains a leading cause of
morbidity and mortality. Therefore, markers for disease
prediction and prognosis are highly desirable for early
diagnosis as well as for helping optimize and personalize
treatment. Recently, miRNAs, short-sequence of non-coding RNAs, have emerged as new biomarkers in the clinical
diagnosis and treatment of ovarian cancer. The dysregulation of miRNAs is involved in the initiation and progression of human cancers including ovarian cancer, and strong
evidence that miRNAs can act as oncogenes or tumor suppressor genes has emerged. The aim of our study is to
evaluate if platinum sensitivity is associated to a specific
pattern of miRNA expression.
Matherials and Methods: We retrospectively selected pts
with histological diagnosis of HGSOC from 2010 to 2019,
stage FIGO IIC-IIIC, treated with a first line platinumbased chemotherapy. PTS were divided into 2 groups: 1)
highly platinum-sensitive (PFI>12months); 2) Partially
platinum-sensitive/platinum-resistant (PFI<12months).
For each pts we evaluated miRNA expression pattern in
formalin-fixed paraffin-embedded samples (primary tumor
or metastasis) using NGS with the Illumina platform.
Results: We collected 6 pts with HGSOC diagnosis for
each group (a total of 12pts). Among the 2500 miRNA
detected using NGS we identified 26 miRNA with a different expression pattern between the 2 groups, in particular:
16 miRNA (miR-3928-3p, miR-501-5p, miR-382-3p,
miR-432-5p, miR-3403p, miR-885-5p, miR-675-3p, miR218-2-3p, miR-218-5p, miR-491-5p, miR-181a-3p,
miR181a-5p, miR-9-3p, miR-9-5p, miR-6832-3p, miR3607-3p) were over-expressed in Group 2 and had a low
expression in Group 1. Vice versa 10 miRNA (miR-4271,

Tumori Journal 106(2S)
miR-6125, miR-205-3p, miR-3180-5p, miR-6255p, miR194-5p, miR-192-5p, miR-215-5p, miR-378j, miR-6860)
had a low expression in Group 2 and a over expression in
Group 1.
Conclusions: Despite the small sample size, this study
showed the correlation between some miRNA and the platinum sensitivity of HGSOC, suggesting that these shortsequences of non-coding RNAs could have a prognostic
and predictive value. Probably in the near future we could
use miRNA expression pattern as a biomarker to select the
most effective treatment for each patient affected by ovarian cancer.

F03
Phenotypic and functional
properties of Natural Killer cells
in patients with ovarian cancer
Provinciali N.1, Pesce S.2, Greppi M.2, Tabellini G.3, Patrizi O.3,
Rampinelli F.4, Obino V.2, Olive D.5, Sivori S.2, Buttiron Webber
T.6, Paleari L.7, Rutigliani M.6, Di Luca M.6, Gorlero F.6, Parolini S.3,
DeCensi A.6, Marcenaro E.2
1

Ente Ospedaliero Ospedali Galliera, Genova; 2Department of Experimental
Medicine (DI.ME.S.), University of Genoa, Genoa; 3Department of Molecular
and Translational Medicine, Brescia, Italy, Brescia; 4Department of Obstetrics
and Gynecology, Spedali Civili of Brescia, Brescia; 5Team Immunity and
Cancer, CRCM, Aix Marseille Univ, CNRS, INSERM, Institut Paoli-Calmettes,
Marseille; 6Ente Ospedaliero Ospedali Galliera, Genoa; 7ALiSa, Liguria Health
Authority, Genoa

Background: Natural Killer cells (NK) can recognize and
kill tumor cells; this cytotoxic activity is regulated by activating and inhibitory signals. Different mechanisms of
tumor escape have been described for cancer. We recently
identified two mechanisms of tumor escape from NK cell
immunosurveillance in ovarian cancer patients: the downregulation of the activating receptor NKp30 mediated by a
soluble form of its ligand B7-H6 (sB7-H6) in the tumor
microenvironment (TME), and the expression on NK cell
surface of the immune checkpoint PD-1, originally identified in T cells. However, little is known about the impact of
these mechanisms on ovarian cancer survival rate and the
application of innovative immunotherapeutic approaches.
Material and methods: We isolated NK from peritoneal/
ascitic fluid (PF) and peripheral blood (PB) of 50 patients
with papillary serous ovarian cancer and from PB of
healthy donors. NK cells have been analyzed for expression of both immune checkpoints, including PD-1, and
activating NK receptors and their role in the modulation of
NK cytotoxicity towards PD-Ls+ tumor cells.
Results: PD-1 expression on PB-NK was shown in 25% of
samples from Human Cytomegalovirus+ otherwise
healthy donors. PD-1 was confined to fully mature NK
cells with low level of activating NK receptors, including
NKp30. PD1+ve NK were also found in PF and PB from



Tumori Journal Abstract Book - October 2020

Table of Contents for the Digital Edition of Tumori Journal Abstract Book - October 2020

Contents
Tumori Journal Abstract Book - October 2020 - Cover1
Tumori Journal Abstract Book - October 2020 - Cover2
Tumori Journal Abstract Book - October 2020 - I
Tumori Journal Abstract Book - October 2020 - II
Tumori Journal Abstract Book - October 2020 - Contents
Tumori Journal Abstract Book - October 2020 - IV
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