Laboratory Animals - June Issue - 291

Hansen et al.

291

` l'avenir, les groupes de recherche devraient
la Prevotella copri et les bacte
´ries filamenteuses segmente
´es. A
donc envisager, pour leurs mode
`les, la pre
´sence ou l'absence d'espe
`ces bacte
´riennes de
´finies figurant sur
une courte liste. Les espe
`ces figurant sur cette liste peut e
ˆtre teste
´es par un se
´quenc¸age d'un bon rapport
cou
ˆt-efficacite
´ ou me
ˆme par une simple approche de PCR multiple, susceptible de ne pas avoir d'incidence
e
´conomique par rapport aux me
´thodes de de
´pistage plus traditionnelles.

Abstract
Bakterien haben im Rahmen von Nagetier-Qualita
¨tssicherungsprogrammen Relevanz, wenn (1) die Tiere
dadurch gefa
¨hrdet sind und (2) das Auftreten bei den Tieren Einfluss auf Tierforschung oder Wohlergehen
der Tiere hat, z. B. weil der Erreger(2A) den klinischen Krankheitsverlauf steuert oder (2B) den Wirt auf
andere Weise beeintra
¨chtigt. Außerdem sind (2C) Zoonosen relevant. Einige international im Rahmen der
Gesundheitsu
¨berwachung von Ratten und Ma
¨usen empfohlene Bakterienarten, z. B. Citrobacter rodentium,
Corynebacterium kutscheri, Salmonella spp. und Streptokokkenpneumoniae, kommen inzwischen weder bei
Ratten oder Ma
¨usen im Labor noch in Tierhandlungen vor, wa
¨hrend andererseits Filobacterium rodentium,
Clostridium piliforme, Mycoplasma spp., Helicobacter spp. und Rodentibacter spp. weiterhin das Risiko einer
Auswirkung auf Tierforschung und Tierschutz bergen, und Streptobacillus moniliformis trotz eines sehr
geringen Risikos als ernstzunehmender Zoonoseerreger gelten du
¨ rfte. Moderne molekulare Techniken
haben gezeigt, dass es je nach Forschungsrichtung ebenso gute Gru
¨nde geben kann, den Koloniezustand
einiger symbiotischer Bakterien zu kennen, die fu
¨ r die Entwicklung spezifischer Nagetier-Modelle wesentlich
sind, wie z. B. Alistipes spp., Akkermansia muciniphila, Bifidobacterium spp., Bacteroides fragilis, Bacteroides
vulgatus, Faecalibacterium prausnitzii, Prevotella copri und segmentierte filamento
¨se Bakterien. In Zukunft
sollten die Arbeitsgruppen daher beru
¨cksichtigen, ob eine Auswahlliste definierter Bakterienarten, die fu
¨r
ihre Modelle relevant sind, vorhanden ist oder nicht. Eine solche Liste kann durch kostengu
¨nstige
Sequenzierung oder auch durch einen einfachen multiplen PCR-Ansatz getestet werden, der im Vergleich
zu herko
¨mmlichen Screening-Methoden kostenneutral sein du
¨rfte.

Resumen
Las bacterias son relevantes en los programas de garantı´a de calidad en roedores si (1) los animales esta
´n en
riesgo y (2) la presencia en los animales marca la diferencia para la investigacio
´n del animal o su bienestar, p.
ej., porque el agente (2A) regula el avance de la enfermedad clı´nica o (2b) impacta en su hue
´sped de otro
modo. Asimismo, (2C) zoonosis son relevantes. Algunas especies de bacterias recomendadas internacionalmente para el control de la salud de ratas y ratones, es decir, Citrobacter rodentium, Corynebacterium
kutscheri, Salmonella spp., y neumonı´ Streptococcus, ya no se ecuentran ni en ratas o ratones de tiendas
de animales o laboratorios, mientras que todavı´a hay un riesgo real de impacto en la investigacio
´n animal y el
bienestar de Filobacterium rodentium, Clostridium piliforme, Mycoplasma spp., Helicobacter spp., y
Rodentibacter spp., mientras que Streptobacillus moniliformis puede ser considerado un agente zoono
´tico
a pesar de tener un riesgo muy bajo. Distintas te
´cnicas moleculares modernas han revelado que puede haber,
dependiendo del tipo de investigacio
´n, igualmente buenos motivos para saber el estado de la colonia de
algunas bacterias comensales que son esenciales para la induccio
´n de modelos de roedores especı´ficos,
como Alistipes spp., Akkermansia muciniphila, Bifidobacterium spp., Bacteroides fragilis, Bacteroides vulgatus, Faecalibacterium prausnitzii, Prevotella copri, y bacterias filamentosas segmentadas. En el futuro, los
grupos de investigacio
´n deberı´an considerar por tanto la presencia o ausencia de una lista final de especies
de bacterias definidas de relevancia para sus modelos. Esta lista puede ser probada mediante una secuencia
de rentabilidad o incluiso un me
´todo PCR mu
´ltiple simple, que es probable que sea de coste neutro en
comparacio
´n a otros me
´todos de escrutinio ma
´s tradicionales.



Laboratory Animals - June Issue

Table of Contents for the Digital Edition of Laboratory Animals - June Issue

Contents
Laboratory Animals - June Issue - Cover1
Laboratory Animals - June Issue - Cover2
Laboratory Animals - June Issue - Contents
Laboratory Animals - June Issue - 214
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Laboratory Animals - June Issue - Cover3
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