JCU - Abstracts of the BAUS 2018 Scientific Meeting - June 2018 - 9

773021

URO

Journal of Clinical UrologyAbstracts

Abstracts

Best Academic Paper Session

Monday 25 June
09:00-10:00
Room 3B
Chairs: Trinity Bivalacqua & John
McGrath
1 - The utility of plasma cell-free DNA
mutations in detecting metastatic recurrence
in patients after radical cystectomy for
bladder cancer
Khetrapal P1, Dong L2, Wong Y2, Tan W1, Rodney S1,
Lamb B1, Briggs T1, Thompson J1, Sridhar A1, Kelly J1,
Feber A1
1University College Hospital Urology Department, London, United
Kingdom, 2UCL Cancer Institute, London, United Kingdom

Introduction and Objective: In routine practice, imaging is used for surveillance of metastatic bladder cancer
for patients after radical cystectomy (RC). With the availability of high-throughput sequencing, there is a growing
interest in utilising non-invasive liquid biopsy biomarkers
to supplement imaging in detecting metastatic disease.
Methods: 80 patients undergoing RC were prospectively
recruited under the UCL/UCLH Biobank for Studying
Health and Disease (Ethics approval (NC06.11) (HTA
License: 12055), and sequential blood samples were collected at baseline and 1, 3, 6 and 12 months post-RC. Postoperative CT findings for all patients were collected to
monitor for metastatic recurrence. For patients with
known recurrence, samples from time of recurrence and
before recurrence were analysed. Cell-free DNA (cfDNA)
was extracted from plasma using QIAamp Circulating
Nucleic Acid Kit. Targeted amplification was performed
using pre-designed duel-indexed primers, and next generation sequencing (NGS) performed using 22 bladder-cancer specific mutations.
Results: cfDNA was isolated from all collected samples,
with a mean of 77.5 ng/ml and IQR 10.9-72.7 ng/ml
(Qubit). Mutations were detectable in cfDNA at baseline

Journal of Clinical Urology
2018, Vol. 11(1S) 9-94
© British Association of
Urological Surgeons 2018
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https://doi.org/10.1177/2051415818773021
DOI: 10.1177/2051415818773021
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for all patients. Additionally, mutations became detectable
in post-cystectomy samples of 5 of 6 patients who subsequently went on to develop metastatic recurrence visible
on CT scanning.
Conclusions: Genomic analysis of plasma cfDNA has the
potential to supplement imaging in the detection of micrometastatic disease, and to select patients for systemic
treatment. Dynamic changes in mutational burden may
represent tumour heterogeneity and could have an application in tracking sub-clonal populations in metastatic
disease.
2 - MIMIC Study: Does the size and location
of ureteric stones have an impact on the
effectiveness of medically expulsive therapy
in improving spontaneous stone passage in
patients presenting with acute ureteric colic?
Assaf N19, Shah T1, Gao C1, O'Keefe A2, Manning T3,
Peacocke A4, Cashman S1, Nambiar A1, Lamb B1,
Cumberbatch M1, Ivin N8, Maw J8, Ali Abdaal C8, Al
Hayek S8, Christidis D9, Bolton D9, Lawrentschuk
N9, Khan S10, Demirel S10, Graham S10, Koschel S11,
Badgery H11, Brennan J11, Wang L12, Nzenza T12,
Ruljancich P12, Begum R13, Hamad S13, Shetty A13,
Swallow D13, Abboudi H14, Jalil R14, DasGupta R14, Ho
C15, Parwaiz I15, Davenport K15, Cameron F16, Shingles
C16, Morrow J17, Curry D17, Young M17, MacKenzie
K18, Reid K18, Bordenave M18, Oyekan A19, Sriprasad
S19, Hayat Z20, Morrison-Jones V20, Laird A21, Sharma
A21, Phipps S21, Ngweso S22, Nyandoro M22, Hayne
D22, Hendry J23, Kerr L23, McIlhenny C23, Harris A24,
Rogers A24, Rodger F25, Docherty E25, Ng G25, Seaward
L25, Abdelmoteleb H26, Hawary A26, Eldred-Evans
D27, Bultitude M27, Stonier T28, Simson N28, SIngh
H28, Hatem E28, Arya M28, Tregunna R29, Ibrahim H29,
McGrath S30, O'Brien J30, Campbell A31, Cronbach P31,
Bdesha A31, Tait C32, Sakthivel A32, Suraparaju L33,
O'Brien J33, Gupta S33, Pankhania R34, Al-Qassim Z34,
Foley R35, Akintimehin A35, Khan A35, Rezacova M36,
Edison E36, Sandhu S36, Nkwam N37, Grice P37, Khan
M37, Kashora F38, Nehikhare O38, McCauley N38, Mason-
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