JCU - Abstracts of the BAUS 2018 Scientific Meeting - June 2018 - 52

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DNA rate. Our results show that there is rarely a sinister cause for retention and propose the following
algorithm.
P5-7 Does chondroitin sulphate play an active
role in barrier formation by normal human
urothelial cell cultures?
Phillips R1, Smith N2, Southgate J3
Hospital, Wakefield, United Kingdom, 2Hull & East
Yorkshire Hospitals, United Kingdom, 3Jack Birch Unit for Molecular
Carcinogenesis, University of York, United Kingdom

1Pinderfields

Introduction: Chondroitin sulphate (CS) has been
described as contributing to the barrier function of the
urothelium by forming a thick glycosaminoglycan (GAG)
layer on the superficial surface. Various papers have suggested that a defect in this layer contributes to chronic
inflammatory uropathies and this forms the basis of medical device therapies.
Materials and Methods: Immunohistochemistry of
human urothelium with well-characterised antibodies to
CS epitopes was used to assess the presence of CS on
urothelium in situ. The role of exogenous CS on barrier
formation was examined during differentiation of normal
human urothelial cell cultures by monitoring transepithelial electrical resistance (TEER).
Results: Immunohistochemical studies supported the
presence of CS within the stroma, but not as a superficial
urothelial GAG layer.
Urothelial cell cultures exposed to chondroitin 4 or 6 sulphate during differentiation formed a tighter maximum
barrier compared to non-treated controls (p=0.0087 and
p=0.0403 respectively, one-way ANOVA, n=9 in each arm,
three individual cell lines). A barrier formed earlier with
chondroitin-6-sulphate compared to control where, by
day 3, there was a statistically significant difference in TEER
readings (p=0.0044, one-way ANOVA, n=6 replicates in
each arm, two individual cell lines).
Conclusion: This study has demonstrated that CS
enhances barrier formation by human urothelial cells in
culture. Intense labelling of CS within the stroma, with
no urothelial presence suggests the mode of action is
unlikely to be a simple supplementation of a GAG layer.
Further studies addressing mode of action are being
pursued.
P5-8 Exploring the use of patients own fat to
stimulate tissue regeneration in the pelvic floor
Mironska E1, Roman S1, Chapple C2, MacNeil S1
of Materials Science and Engineering, University of
Sheffield, United Kingdom, 2Royal Hallamshire Hospital, Sheffield,
United Kingdom
1Department

Journal of Clinical Urology 11(1S)
Introduction: Our aim is to develop a new biodegradable material consisting of polylactic acid (PLA) scaffold
augmented with patients' adipose derived mesenchymal
stem cells (ADMSCs). Synthetic, transvaginal mesh made
of polypropylene (PPL) has been the mainstay of pelvic
organ prolapse surgery but is now recognised to lead to
severe complications in a significant proportion of woman.
Therefore, there is an urgent need for new materials for
use within the pelvic floor. An ideal material would be
strong enough to provide support to prolapsed pelvic
organs but not so stiff as to induce erosion through native
tissues (as currently occurs with PPL). For tissue integration and remodelling of patient's tissues it should also promote angiogenesis, reduce inflammation and increase
collagen production.
Materials and Methods: We used electrospun PLA which
provides good mechanical support and degrades slowly without provoking a strong inflammatory response. ADMSCs
have been shown to promote angiogenesis and reduce
inflammation while promoting connective tissue regeneration. Using cells harvested from donated human fat, we have
isolated a cell rich stromal vascular fraction (SVF) containing
both ADMSCs and other progenitor cells. The SVF can be
harvested both enzymatically and mechanically.
Results: We have developed a biodegradable polylactic
acid (PLA) electrospun scaffold containing pockets into
which ADMSCs can be placed. We report that ADMSCs
attach and proliferate well within PLA pockets, remaining
viable and metabolically active.
Conclusion: We report the first steps to achieving a
material which can be combined with patients' cells for
supporting regeneration of the pelvic floor in one single
operation.
P5-9 Detailed characterisation of severe
incontinence after robotic-assisted radical
prostatectomy using urodynamics and patient
reported outcome measures
Mackenzie K1, Davis J1, Harding C1, Aning J1
Upon Tyne Hospitals NHS Foundation Trust, United
Kingdom
1Newcastle

Introduction: Urinary incontinence after roboticassisted radical prostatectomy (RARP) can ruin men's
quality of life. Few studies have performed detailed analysis
of post-RARP incontinence in men considering having surgery to improve their leakage. This study investigated the
urodynamic and patient-reported outcome measure
(PROM) data of men considering surgery for post-RARP
incontinence in a single UK centre performing RARP and
incontinence surgery.
Methods: All men referred with post-RARP incontinence
to a specialist clinic between December 2012 and October

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